Fig. 7

Inactive mTORC1 prevents the nuclear localization of STAT3 in macrophages. a Nuclear localization of STAT3 was prevented in cells treated with rapamycin. Scale bars represent 10 μm. The nuclear fluorescence intensity of STAT3 was shown. b Nuclear localization of STAT3 was prevented in cells treated with Stattic. Scale bars represent 10 μm. The fluorescence intensity of nuclear STAT3 was graphed. c The model of mTORC1 regulating pyroptosis through nuclear localization of STAT3 and pyroptosic gene expression in human macrophage. STAT3 is likely a negative regulator of pyroptosic gene expression. mTORC1 inhibition by rapamycin or STAT3 inhibition by Stattic prevents the nuclear localization of STAT3, upregulates pyrotosic gene expression, triggering human macrophage pyroptosis