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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages

Fig. 1

Staphylococcus aureus invades macrophages. S. aureus infected macrophages for 3 h, and then, the macrophages were cultured in medium supplemented with antibiotics and lysozyme to kill and lyse the extracellular bacteria. The intracellular bacteria were detected by staining and laser confocal microscopy and TEM. a The intracellular S. aureus (green) stained with CFSE was observed by laser confocal microscopy; macrophages nuclei were co-stained with DAPI (blue), and actin was stained with phalloidin (red). Scale bars represent 50 μm. b Bacteria were attached to the cell membrane and engulfed, based on micrographs obtained by TEM; several important observations are magnified. Red arrows indicate S. aureus. Scale bars represent 2 μm. a′ Control, non-infected human macrophages. b′ The bacteria were located predominantly in the plasma membrane; macrophages were allowed to engulf bacteria and initiate phagocytosis. c′ S. aureus was engulfed by macrophages via the formation of typical phagocytic cups

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