Fig. 1

The role of MPs in pulmonary vascular leakage and lung injury after I/R. a–d The change of MP concentration in blood in hemorrhage-transfusion model (Hemo-Trans) (a, b) and ischemia/reperfusion model (I/R) (c, d), MPs were marked with annexin V antibodies. e, f The change of pulmonary vascular permeability reflected by leakage of Evans blue 30 min after injection (iv) in I/R rats. g, h The status of inflammatory cells infiltration in lung after I/R. The numbers of inflammatory cells were determined in 10 randomly selected nonoverlapping fields at 400 × magnification in respective sections of the individual rat lung. i, j Effects of intravenous administration of NC-MPs and I/R-MPs on pulmonary vascular permeability reflected by the leakage of Evans blue. k, l Effects of NC-MPs and I/R-MPs on inflammatory cells infiltration in lung. The data are the mean ± SD of n experiments (n = 8). Sham sham-operated group, NC-MP MPs derived from the blood of normal rats; I/R-MP MPs derived from the blood of ischemia/reperfusion rats. **P < 0.01 versus N or sham group