Fig. 7
From: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
LA facilitates the fibrosis through upregulating the expression of Cadherin-11. a Go enrichment analysis showed pathways related to Cadherin-11 in LA-treated CD115(+) precursors. b RT-PCR analysis detected the mRNA levels of specific fibrotic markers in bone marrow at different timepoints after intratibially injection of MC-38 cells. c-g The primary CD115(+) precursors were isolated and transfected with Cadherin-11 siRNA or negative controls, then the cells were treated by LA for 72 h. The mRNA expression of each fibrotic marker was detected by RT-PCR. h After injecting MC-38 cells, LA was injected intraperitoneally for 5 days and Cadherin-11 siRNA was injected every 2 days. Then the primary CD115(+) precursors were isolated and fixed. Immunofluorescence analysis revealed the expression of Col5a2 in CD115(+) precursors sorted from mice in each group (left) and the quantification of percentage of Col5a2(+) cells (right) (Scale bar = 50 μm). *p < 0.05, **p < 0.01, ***p < 0.001