Fig. 2
From: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
LA induces the expression of CXCL10 in osteoclast precursors to promote the formation of osteolytic formation. a Cellular pathways enriched in RNA-Seq analysis and scatterplot illustration showed differently expressed genes in CD115(+) precursors in LA-treated group comparing with that genes expressing in control group. b RT-PCR analysis showed the mRNA level of Cxcl10 in CD115(+) precursors treated by LA for 72 h comparing with treated by PBS. c RT-PCR analysis detected the mRNA expression of Cxcl10 in freshly sorted CD115(+) precursors from bone marrow after injection of MC-38 cells at 0 day and 10 day with/without treatment with LA. d Trap staining revealed number of Trap(+) cells at 14 day after injection of CRC cells and administration of LA with/without CXCL10 neutralizing antibody, and the quantification of the number of osteoclasts (Scale bar = 50 μm). e Safranin O and fast green staining showed the trabecular area at 14 day after injection of MC-38 cells and treated by LA with/without CXCL10 neutralizing antibody, and quantification of percentage of trabecula area (Scale bar = 50 μm). *p < 0.05, **p < 0.01, ***p < 0.001