Fig. 1
From: Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
LA facilitates the progression of bone metastasis from CRC. a The concentration of LA in MC-38 conditional media was tested by ELISA assay after cultured for 3 days. b The concentration of LA in bone marrow at specific timepoints after injection of MC-38 cells was tested by ELISA assay. c Safranin O and fast green staining was performed to detect the trabecular area between PBS treated group and LA treated group at 2 weeks post injection of MC-38 (Scale bar = 50 μm). d Quantification of trabecular area in (c). e TRAP staining showed the osteoclast accumulation in trabecular area in PBS-treated group and LA-treated group at 2 weeks after injection of MC-38 (Scale bar = 50 μm). f Quantification of osteoclast numbers in (e). g Survival analysis in LA-treated group vs. control group in bone metastasis model of CRC. *p < 0.05, **p < 0.01, ***p < 0.001