Fig. 3

RAGE knockdown suppresses LPA induced downstream pathways in mammary cancer cells. a, b Levels of RAGE in mammary cancer cells (MDA MB-231 and MCF-7, respectively) after shRNA mediated RAGE silencing. c, d Levels of p-ERK/ERK, and p-PKB (Ser473)/PKB, p-STAT3/STAT3 and GAPDH in mammary cancer cells (MDA MB-231 and MCF-7, respectively) treated with LPA for the indicated time points after shRNA mediated RAGE silencing. e Quantification of cell proliferation assay of control and RAGE silenced A549 with LPA post PKB inhibitor treatment (LY294002). f F-actin polymerization in control and RAGE shRNA MDA MB-231 and MCF-7 cells cultured in medium with or without LPA for 6 h (green, F-actin; blue, DAPI). g Representative images and quantifications of transwell invasion assays of control and RAGE shRNA treated MDA MB-231 and MCF-7 cells after LPA treatment. h, i Representative images and quantification of colonies of MDA MB-231 and MCF-7 cells treated with control shRNA and RAGE shRNA and then grown in medium with or without LPA for 10 days. (For all experiments, data are means ± SD., n = 3–7. * ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001)