Fig. 9

Depletion of clathrin and dynamin enhances expression of LTβR target genes in A549 cells. mRNA levels of NF-κB target genes were analyzed by qRT-PCR in A549 cells transfected with siRNAs targeting clathrin (CHC, two oligonucleotides denoted with consecutive numbers) (a, b), dynamin-1/2 (three combinations of oligonucleotides targeting dynamin-1 and dynamin-2, see Methods) (c, d) and with relevant non-targeting siRNAs and stimulated with Ago for 2 h. Values are presented as a fold change vs unstimulated averaged non-targeting controls (AvCtrl), set as 1. Data represent the means ± SEM, n ≥ 3; ns - P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 by one sample t test. Tables present the fold change of expression of the indicated genes in stimulated vs unstimulated cells transfected with different combinations of siRNAs, targeting clathrin (b) and dynamin-1/2 (d), and non-targeting controls