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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor

Fig. 5

Stimulation of LTβR with ligands preferentially activates NF-κB signaling. Lysates of A549 cells stimulated with: Ago, LTα1β2 and LIGHT for different time periods were analyzed by Western blotting with antibodies against the indicated proteins to assess activity of the NF-κB pathway (a) and JNK-dependent AP-1 pathway; P-JNK - phospho-JNK; analyzed bands marked with asterisks (b). Graphs show densitometric analysis of abundance of the indicated proteins, normalized to vinculin, used as a loading control. Values are presented as a fold change vs unstimulated control (−), set as 1. Data represent the means ± SEM, n ≥ 3; ns - P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 by one sample t test. Representative blots are shown

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