Table 4 Characterization of GCEC by CD
Factor | α-helises (%) | β-strands (%) | Turns (%) | Ua (%) | ||||
|---|---|---|---|---|---|---|---|---|
Ra | Db | Σ | Ra | Db | Σ | |||
– | 0.5 ± 1.6 | 4.9 ± 2.1 | 5.4 ± 3.7 | 22.7 ± 3.5 | 9.2 ± 2.5 | 31.9 ± 6.0 | 13.8 ± 1.5 | 49.0 ± 5.5 |
15% TFE | 8.7 ± 3.2 | 8.9 ± 1.5 | 17.6 ± 4.7 | 16.9 ± 1.6 | 9.7 ± 2.1 | 26.2 ± 3.7 | 19.4 ± 2.0 | 36.4 ± 2.0 |
30% TFE | 14.9 ± 6.2 | 12.8 ± 2.3 | 27.2 ± 8.5 | 10.7 ± 3.0 | 7.7 ± 2.8 | 18.4 ± 5.8 | 19.1 ± 3.4 | 34.3 ± 1.1 |
1 M GdmCl | 0.2 ± 2.5 | 1.8 ± 3.7 | 2.0 ± 6.2 | 14.1 ± 3.4 | 8.7 ± 3.1 | 22.8 ± 6.5 | 12.8 ± 5.4 | 62.3 ± 4.8 |
2 M GdmCl | 0.0 ± 4.0 | 2.0 ± 5.2 | 2.0 ± 9.2 | 11.5 ± 5.1 | 6.4 ± 5.7 | 17.9 ± 10.8 | 10.0 ± 6.0 | 68.1 ± 5.2 |
4 M GdmCl | – | – | – | – | – | – | – | 79.8 ± 7.8 |