Fig. 2
Hydrodynamic properties of GCEC. a Analytical SEC of GCEC performed on a Superdex200 10/300 GL column. The graph presents the elution volume of GCEC in a concentration of 1 mg/ml. The inset represents the standard curve determined with standard proteins (black dots). The green circle corresponds to the GCEC.b AUC analysis. The graph presents the sedimentation coefficient distributions c(S) for the GCEC at three concentrations: 0.30 mg/ml (black solid line), 0.18 mg/ml (grey dashed line), and 0.07 mg/ml (black dotted line). All data were collected at 230 nm. c The representative example of the GCEC sedimentation profile. Selected experimental (circles) and fitted SV profiles (solid lines for GCEC at 0.30 mg/ml) are shown. d The relationship between the hydrodynamic radii (RS) and the relative MMs determined for four globular proteins states (dark grey) and two IDPs states (light grey) [51, 52]. The globular proteins states are: native proteins (N, tringles), molten globules (MG, squares), pre-molten globules (PMG, diamonds), and 6 M GdmCl-unfolded proteins (coil, circles). The two IDPs states are: coil-like (circles), and PMG-like (diamonds). The theoretical value for GCEC (green dot) and experimental values (SEC – red dot or AUC yellow dot) are shown