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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: JARID1B promotes colorectal cancer proliferation and Wnt/β-catenin signaling via decreasing CDX2 level

Fig. 5

CDX2 was required for JARID1B-mediated Wnt/β-catenin signaling in CRC cells. a mRNA levels of CDX2 were detected by qRT-PCR. The knockdown of CDX2 expression dramatically inhibits the increase of CDX2 expression in DLD-1-shJARID1B cells. b Western blot analysis showed that the knockdown of CDX2 expression markedly rescused the increase of GSK-3β, Axin2 and p-β-catenin expression and the decrease of β-catenin, c-MYC and cyclinD1 expression induced by JARID1B knockdown. c Ubiquitinated β-catenin levels when JARID1B and CDX2 knockdown in DLD-1 cells. d Silencing CDX2 attenuated the loss of downregulating JARID1B on Wnt/β-catenin as observed by TOP-Flash luciferase assay. e The upregulation of CDX2 mRNA expression markedly inhibited the decrease in CDX2 expression observed in JARID1B-overexpression LOVO cells. f Western blot analysis showed that the levels of JARID1B overexpression and CDX2 upregulation and their effects on GSK-3β, Axin2, β-catenin, c-MYC and cyclinD1 expression in LOVO cells. g Ubiquitinated β-catenin levels when JARID1B and CDX2 over-expressed in LOVO cells. h TOP-Flash luciferase reporter assay showed the overexpression of ectopic CDX2 attenuated the increase in Wnt/β-catenin activitiy in JARID1B-overexpression LOVO cells.*p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X