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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: JARID1B promotes colorectal cancer proliferation and Wnt/β-catenin signaling via decreasing CDX2 level

Fig. 3

Stable knockdown of JARID1B repressed CRC proliferation via Wnt/β-catenin signaling pathway. a Gene set enrichment analysis of JARID1B based on TCGA COAD RNA Seq dataset showed that JARID1B expression was positively correlated with Wnt/β-catenin signaling pathway. b Western blot detected JARID1B, β-catenin c-MYC and cyclinD1 expression in DLD-1 cells transfected with shNC or shJARID1B. c The total and nuclear protein levels of β-catenin were assessed by western blotting in DLD-1 cells transfected with shJARID1B or shNC. d The inhibitive effect of JARID1B downregulation on Wnt/β-catenin pathway was detected by TOP-Flash luciferase reporter assay. e Western blot analysis showed the effects on c-MYC, cyclinD1 expression in DLD-1 cells when both JARID1B silencing and β-catenin restoration. f TOP-Flash luciferase reporter assay showing that β-catenin overexpression rescued the decreased Wnt/β-catenin pathway activity of DLD-1-shJARID1B cells. g, h, i EdU and in-vivo tumor formation assays showed that over-expression of ectopic β-catenin significantly rescued cells proliferation in DLD-1- shJARID1B cells. *p < 0.05

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