Table 1 Sequences of primers used to analyze ECs gene expression
Gene | Gene ID | Sequence | Fragment length (bp) |
|---|---|---|---|
Angpt1 | 89807 | F: 5′ GCTAACAGGAGGTTGGTGGT 3′ R: 5′ CACTTTATCCCATTCAGTTTTCCA 3’ | 102 |
Col4a1 | 290905 | F: 5’ GAACGAAAGGGACACGAGGAC 3′ R: 5′ CAAGCCAGGAGGACCGAGTG 3’ | 158 |
Pecam1 | 29583 | F: 5’ CCCTGTCCCCACCCAAAG 3′ R: 5′ ACCTCCTCTCACCTCCCAAA 3’ | 173 |
Vegfa | 83785 | F: 5’ CTTCTTCCACCACTGTGTCT 3′ R: 5′ GCCTCAGGACATGGCACTAT 3’ | 200 |
Mmp2 | 81686 | F: 5’ CCCTCCCCTGATGCTGATAC 3′ R: 5′ CTGTCCGCCAAATAAACCGAT 3’ | 157 |
Rplp0 | 64205 | F: 5’ CACTGGCTGAAAAGGTCAAGG 3′ R: 5′ GTGTGAGGGGCTTAGTCGAA 3’ | 187 |
Gapdh | 24383 | F: 5’ GGCATTGCTCTCAATGACAA 3′ R: 5′ TGTGAGGGAGATGCTCAGTG 3’ | 213 |
Hprt1 | 24465 | F: 5’ AGCCTAAAAGACAGCGGCAA 3′ R: 5′ GGCCACAGGACTAGAACGTC 3’ | 87 |