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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling

Fig. 4

Effect of the inhibition of CGRP and SP on ECs transcriptional profiles. ECs were cultivated for 7 days in the microfluidic devices in presence of sensory neurons. Endothelial cells were either not treated (EGM-2MV) or treated with CGRP antagonist (BIBN4096BS), SP antagonist (SR140333) or both, at a concentration of 10 μM each. Total RNA was extracted and gene expression of VegfA, Angpt1, Col4 and Mmp2 was analyzed by RT-qPCR. CGRP and SP antagonists downregulate ECs gene expression of Angpt1, VegfA and Col4, both separately and together. Mmp2 gene expression is only significantly downregulated when ECs were treated with both antagonists. (n = 6 microfluidic devices, One-way ANOVA followed by post hoc Dunnett’s test, * p < 0.05, ** p < 0.01, *** p < 0.001)

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