Fig. 5

SOCS3 expression is a secondary response to H. pylori-induced TNFα. After 1 h of infection withH. pylori at MOI = 5, DCs were harvested and mRNA expression was analyzed by qRT-PCR.a, b 1 h prior to infection with H. pylori strain P12 WT (orange) or ΔCagPAI (green), Brefeldin A (1 μg/ml) was added to the cells. H. pylori was added to the cells at MOI = 5 and SOCS3, IL6 and TNFα mRNA expression was evaluated after 1 h. Two experiments comprising 6 individual donors (N = 6) are shown. Dots represent individual donors; mean ± SD is shown. c Cells were treated with a specific TNFα neutralizing antibody (100 ng/ml) before infection with H. pylori strain P12 WT or stimulation with TNFα (10 ng/ml). After 1 h, SOCS3 mRNA expression was assessed. One experiment comprising 5 individual donors (N = 5) is shown. Dots represent individual donors; mean ± SD is shown. For statistical analysis, one-way ANOVA with Tukey’s post-hoc test was performed. *p < 0.05, **p < 0.01