Fig. 4

H. pylori triggers early phosphorylation of p38 which correlates with SOCS3 expression. Day-7 DCs were re-plated in fresh medium, H. pylori strain P12 was harvested in PBS and added to the cells at MOI = 5. a Protein levels and phosphorylation of p38, STAT1 and STAT3 were analyzed at the indicated time points by western blotting. One representative experiment out of two is shown. b DCs were infected with H. pylori strain P12 wt or ΔCagPAI, and p-p38 levels were detected after 30 min. Three individual donors are shown. c DCs were infected with H. pylori strain P12 wt or ΔCagPAI, and SOCS3 mRNA expression was analyzed after 1 h. Dots represent individual donors, mean ± SD of eight individual donors is shown. For statistical analysis, one-way ANOVA with Tukey’s post-hoc test was performed. ***p < 0.001