Fig. 3

H. pylori-inducedSOCS3 expression is mediated by p38 and independent of TLR2/4 and JAK kinase. After 1 h of infection with H. pylori at MOI = 5 or stimulation with LPS, DCs were harvested and SOCS3 mRNA expression was analyzed by qRT-PCR. a 20 min prior to infection with H. pylori or stimulation with LPS (1 ng/mL), blocking antibodies for TLR2 (1 μg/mL) and TLR4 (1 μg/mL) were added to the cells (αTLR2/4). H. pylori strain P12 was added to the cells at MOI = 5 (orange). LPS was added at 1 ng/mL (blue). One experiment comprising 3 individual donors is shown. (N = 3) b, c Specific inhibitors of JAK kinase (1 μg/ml) or p38 (SB203) (10 μM) were added to the respective samples. Dots represent individual donors; mean ± SD is shown. b Four experiments comprising 9 donors (N = 9) and c three experiments comprising 6 donors (N = 6) are shown. For statistical analysis, one-way ANOVA with Tukey’s post-hoc test was performed. *p < 0.05, **p < 0.01