Fig. 5

Silencing of hnRNPA1 stimulated lipid accumulation in muscle cells. (a) Real-time PCR analysis of hnRNPA1 expression in muscle cells treated with si-hnRNPA1. (b) Western blot analysis revealed that hnRNPA1 protein expression level was suppressed by si-hnRNPA1. Representative gel images are presented in the left panel, and quantitative data are in the right panel. TG contents(c) and DAG contents (d) in muscle cells in the absence or presence of FFA after hnRNPA1 knockdown. (e) Representative tracings of OCR measured in the hnRNPA1 knockdown and control muscle cells. Basal mitochondrial respiration (f), maximal mitochondrial respiration (g), ATP production (h) and proton leak (I) of si-hnRNPA1 and si-Con transfected muscle cells. (j) Representative images of Nile red staining assays in muscle cells of hnRNPA1 silencing. (k) hnRNPA1 knockdown decreased the protein levels of glucose and lipid metabolic genes in muscle cells. Representative gel images are presented in the left panel, and quantitative data are in the right panel. Quantification was based on 3 independent transfection/infection experiments. Numbers were the mean ± SD. ***p < 0.001, **p < 0.01, *p < 0.05, n.s, not significant