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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: LncRNAH19 improves insulin resistance in skeletal muscle by regulating heterogeneous nuclear ribonucleoprotein A1

Fig. 4

Validation of interaction between hnRNPA1 and H19. a Flow diagram of RNA pull-down assay. b Silver staining result of RNA pull-down assay revealed H19 interacted with hnRNPA1 in normal C2C12 cell lines. c Western blotting identified hnRNPA1 bound with H19. d RNA immunoprecipitation (RIP) revealed that hnRNPA1 was bound with H19. e RIP identified that hnRNPA1 was bound with CPT1b, CD36, PGC1a. Quantification was based on 3 independent transfection/infection experiments. Numbers were the mean ± SD. ***p < 0.001, **p < 0.01, *p < 0.05, n.s, not significant

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