Fig. 2

H19 regulates lipid metabolism in skeletal muscle cells. (a) Real-time PCR analysis of H19 expression in muscle cells treated with ad-H19. DAG contents(b) and TG contents (c)in muscle cells in the absence or presence of FFA after H19 overexpression. (d) Real-time PCR analysis of H19 expression in muscle cells treated with si-H19. DAG contents(e) and TG contents (F)in muscle cells in the absence or presence of FFA after H19 knockdown. (g) Representative images of Nile red staining assays in muscle cells of H19 overexpression or silencing. (h) Representative tracings of OCR measured in the H19 overexpression and control muscle cells. Maximal mitochondrial respiration (i), basal mitochondrial respiration (j), ATP production (k) and proton leak (l) of ad-H19 and ad-GFP infected muscle cells. (M) Representative tracings of OCR measured in the H19 knockdown and control muscle cells. Maximal mitochondrial respiration (n), basal mitochondrial respiration (o), ATP production (p) and proton leak (q) of si-H19 and si-Con transfected muscle cells. Quantification was based on 3 independent transfection/infection experiments. Numbers were the mean ± SD. ***p < 0.001, **p < 0.01, *p < 0.05