Fig. 4

C212, in contrast to chemotherapy drugs, effectively eliminates dormant leukemia cells. a, b Serum starvation-induced quiescence and slow-growing state. HL60 (a) and K562 (b) cells were deprived of serum for 12 and 36 h, respectively, to induce quiescence/slow-growth; EdU (2 μM) was added to the medium and cells were further incubated for 43 h (a) and 24 h (b), followed by a Click-iT assay to assess EdU incorporation in cells. Growing: control cells not deprived of serum but otherwise under the same treatment. Error bar, SEM (n = 2). c-l Growing and serum starvation-induced (as in a andb) quiescent cells (HL60,c, e-h; K562,d, i-l) were treated with C212, paclitaxel, topotecan, doxorubicin, and midostaurin at the indicated doses for 48 h and subjected to MTS assay. Error bar, SEM (n = 3)