Fig. 5

Effect of thrombospondin-1 on microvesiculation of human red blood cells. Gating strategy in imaging flow cytometry analysis (a). The Dark Field (DF) Scattering intensity (SSC, Ch12) Area and Aspect Ratio Features (including two Bright Fields (BF) (BF1, Ch01 or BF2, Ch09) were used to differentiate RBC, ghost, and MP subpopulations. Percentages of RBC (gray), ghost (white), and MPs (black) in RBC samples incubated for 48 h in Ringer’s solution at 37 °C (b). Ratio of CD47⁺ CD235a⁺ MPs to RBCs after 48-h incubation at 37 °C in the absence (Control) or presence of TSP-1 in Ringer’s solution (n = 6; c). Gray lines indicate means. *indicates significant difference (P < 0.05) from the absence of TSP-1