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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer

Fig. 2

Global SUMOylation increases in TamR-BCa. a SUMO transcripts are significantly higher in TamR-7 BCa cells. Real-time PCR was performed using TamR-7 and parental MCF-7 cells. Graph represents fold-changes (2ΔΔCt values) in gene expressions of 3 independent experiments and statistical significance in raw ΔCt values using Student’s t-test. b-c Tam-treated HR+ BCa patients expressing high levels of AR and SUMO isoforms show high probability for developing metastasis. Plots for: b HR + -BCa patients; (c) Tam-treated HR+ BCa patients illustrate 10-year DMFS. HR and log-rank P values are enlarged in dotted box. d-e Western blot analysis show enhanced global SUMO2/3 conjugates in acquired and intrinsic TamR-BCa cell lines; SUMO conjugates are marked by asterisks. e Blots from (D) were analyzed by densitometry using ImageJ from at least two independent experiments. Overall SUMO-1 or SUMO-2/3 conjugates were normalized to GAPDH and graphed as mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparison test was performed to compare between groups and statistical significance is shown on the graph

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