Fig. 5

NF-κB and JAK/STAT-dependent regulation of activation and migration of HSCs. (a and b) The iPSCs derived-HSCs were incubated with and without isolated MM and ZZ plasma derived-EVs and the protein expression of the phospho-IKK, total IKK, nuclear p65, nuclear p50, cytoplasmic STAT1 and JNK, and nuclear phospho-STAT-1 and phosphor-JNK were determined during 24 h of incubation by Western blot analysis. GAPDH protein level was used as loading control. The assays were performed at least three times with similar results. c Blot quantification bar graphs. d Wound-healing and e cell proliferation assays of HSCs treated with or without EVs derived from MM and ZZ plasma samples or PBS control during 24 h of incubation. f and g LX-2 cells were incubated with and without isolated MM and ZZ plasma derived-EVs and the expression of CXCL10 and CXCR3 were measured using real-time PCR after 24 h of incubation, *p < 0.05, **p < 0.005, ***p < 0.0005