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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo

Fig. 4

Plasma derived-EVs from AATD individuals activate hepatic stellate cells in vitro. a Fluorescent microscopic images demonstrating interaction of DiO-labeled EVs with recipient HSCs. Images were taken 1 h after EVs were added to the culture medium. Images were captured 20× objective. Flow cytometric analysis confirmed a population of green fluorescence positive cells present in EV-fed groups. b Representative phase contrast images of morphology of quiescent stellate cells incubated with PBS (top row), c (middle row) and ZZ plasma derived-EVs (bottom row) during 24 h of incubation. c LX-2 cells were incubated with and without isolated MM and ZZ plasma derived-EVs and the expression of α-SMA and Col1a1 were measured using real-time PCR after 24 h of incubation. **p = 0.0041 d Immunofluorescence images of the expression levels of α-SMA in LX-2 cells incubated with and without isolated MM and ZZ plasma derived-EVs followed by α-SMA fluorescent intensity. Green fluorescence indicates LX-2 cells that express α-SMA and blue spots indicate nuclei stained with DAPI (Images were captured 40× objective). **p = 0.0020

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