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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo

Fig. 1

Isolation and characterization of plasma EVs. a Ultra-centrifugation was used to isolate EVs from the plasma of normal (MM) and AATD (ZZ) individuals; b NTA was performed on plasma EVs purified by ultracentrifugation to determine their concentration (upper panel) or size (nm) (lower panel) in individual groups of MM and ZZ (26 individuals per group); c EVs were characterized by TEM; d Western blot analysis of EVs using anti-CD 63 (left), anti-CD81 (middle), and anti-TSG 101 antibodies (right). Graphs show quantification of immunoreactive signals by scanning, normalized to the number of the EVs, *p = 0.0013

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Cell Communication and Signaling

ISSN: 1478-811X