Fig. 3

Icariside I has no effect on other stimuli-induced NLRP3 inflammasome activation and also fails to upregulate the activation of non-canonical NLRP3 inflammasome, as well as AIM2 and NLRC4 inflammasomes. a, d Western blot analysis of caspase-1 (p20) and IL-1β in SN of LPS-primed BMDMs treated Icariside I (20 μM) and then stimulated with ATP, nigericin, poly(I:C), SiO2, poly (dA:dT), and Salmonella or Pam3CSK4-primed BMDMs treated with Icariside I (20 μM) and then stimulated with LPS, and Western blot analysis of pro- IL-1β, caspase-1 (p45), NLRP3 and ASC in WCL. b-c, e-f ELISA of IL-1β (b, e) and the release of LDH (c, f) in SN. RLUs, the relative light units. Coomassie blue staining was used as the loading control in the SN a, d.  Data are represented as the mean ± SD from at least three biological samples. The significance of the differences was analyzed using an unpaired Student’s t-test: *P < 0.05, **P < 0.01 vs. the control