Fig. 2

Icariside I promotes NLRP3 inflammasome activation stimulated by ATP and nigericin. a-c Caspase-1 activity (a) and ELISA of IL-1β (b), the release of LDH (c) in SN from LPS-primed BMDMs treated with various doses of Icariside I and then stimulated with ATP. (d) Western blots of SN and WCL  from LPS-primed BMDMs treated with various doses of Icariside I and then stimulated with ATP. e Western blots of SN and WCL from LPS-primed BMDMs treated with various doses of Icariside I before nigericin stimulation. f-h Caspase-1 activity (f), ELISA of IL-1β (g) and the release of LDH (h) from LPS-primed BMDMs treated with various doses of Icariside I and then stimulated with nigericin. RLUs, the relative light units. Data are represented as the mean ± SD from at least three biological samples. ^##P < 0.01, ^###p < 0.001 vs. the control group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the LPS plus ATP or nigericin group