Fig. 1

Effect of the main compositions from EF on NLRP3 inflammasome activation. a, b Caspase-1 activity (a) and ELISA of IL-1β (b) in supernatants (SN) from LPS-primed BMDMs treated with MSU (200 μg/ml) or the main compositions from EF for 4 h. c Western blots of SN and whole cell lysates (WCL) from LPS-primed BMDMs treated with the main compositions from EF(20 μM) and then stimulated with ATP(5 mol/L). d-f Caspase-1 activity (d), ELISA of IL-1β (e) and the release of LDH (f) in SN from LPS-primed BMDMs treated with the main compositions from EF (20 μM) and then stimulated with ATP. RLUs, the relative light units. Data are represented as the mean ± SD from at least three biological samples. ^##p < 0.01,^###p < 0.001 vs. the control group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the LPS plus ATP group