Fig. 7
From: Non-canonical Fzd7 signaling contributes to breast cancer mesenchymal-like stemness involving Col6a1
Col6a1 maintains BC cell mesenchymal phenotype and stemness. a and b Expression of Vimentin, E-cadherin, Slug and Zeb1 was detected in MDA-MB-231 cells (a) and Hs578T cells (b) transfected with shCtrl or shCOL6A1 by Western blot. c and d Migration (c) and invasion (d) of Hs578T cells transfected with shCtrl or shCOL6A1 were analyzed by Wound healing or Transwell. e and f Mammosphere formation in MDA-MB-231 cells (e) and Hs578T cells (f) transfected with shCtrl or shCOL6A1 was shown. g and h The fraction of Lgr5+ subpopulation in MDA-MB-231 cells (g) and Hs578T cells (h) transfected with shCtrl or shCOL6A1 was determined by flowcytometry. All experiments were carried out three times. Data are expressed as Mean ± s.e.m