Fig. 6
From: Non-canonical Fzd7 signaling contributes to breast cancer mesenchymal-like stemness involving Col6a1
Col6a1 is implicated in Fzd7 signaling. a and b Interrogation of CCLE database (a) and GSE12777 database (b) showed the correlation of FZD7 and WNT5B with COL6A1 in BC cell lines. c Interrogation of GSE2603 database showed the correlation of FZD7 and WNT5B with COL6A1 in BC tissues. d Expression of Fzd7 and Col6a1 in BC tissues was detected by Immunohistochemistry. #1, #2, #3 and #4 are representative specimens. e Expression of Col6a1 was detected in MDA-MB-231 cells transfected with shCtrl or shFZD7 by Western blot. f Expression of Col6a1 was detected in MDA-MB-231 cells transfected with shCtrl or shWNT5B by Western blot. g and h Expression of Col6a1, Wnt5a/b, IL-6, p-Stat3, Smad3 and Yap1 was detected in MDA-MB-231 cells (g) and Hs578T cells (h) transfected with shCtrl or shCOL6A1 by Western blot. All experiments were carried out three times