Fig. 5
From: Non-canonical Fzd7 signaling contributes to breast cancer mesenchymal-like stemness involving Col6a1
Wnt5b is involved in BC cell mesenchymal phenotype and stemness. a and b Expression of Vimentin, E-cadherin, Slug and Zeb1 was detected in MDA-MB-231 cells (a) and Hs578T cells (b) transfected with shCtrl or shWNT5B by Western blot. c and d Migration (c) and invasion (d) of Hs578T cells transfected with shCtrl or shWNT5B were analyzed by Wound healing or Transwell. e and f Heat map generated from CCLE database (e) and GSE12777 database (f) demonstrated the correlation of WNT5B with stemness-related genes in human BC cell lines. g Mammosphere formation in MDA-MB-231 cells transfected with shCtrl or shWNT5B was shown. h The fraction of Lgr5+ subpopulation in MDA-MB-231 cells transfected with shCtrl or shWNT5B was determined by flowcytometry. All experiments were carried out three times. Data are expressed as Mean ± s.e.m