Fig. 4
From: Non-canonical Fzd7 signaling contributes to breast cancer mesenchymal-like stemness involving Col6a1
Fzd7 modulates non-canonical Wnt pathways. a Interrogation of databases showed the correlation of FZD7 with WNT5 in BC cell lines (CCLE and GSE12777) and tissues (GSE2603). b and c Co-IP test was performed to verify the binding of Wnt5a/b to Fzd7 in MDA-MB-231 cells (b) and Hs578T cells (c). d Expression of Wnt5a/b, IL-6, p-Stat3, Smad3 and Yap1 was detected in MDA-MB-231 cells transfected with shCtrl or shFZD7 by Western blot. e Expression of Wnt5a/b, IL-6, p-Stat3, Smad3 and Yap1 was detected in MCF7 cells transfected with control vector or FZD7 overexpression vector by Western blot. f and g Expression of Wnt5a/b, IL-6, p-Stat3, Smad3 and Yap1 was detected in MDA-MB-231 cells (f) and Hs578T cells (g) transfected with shCtrl or shWNT5B by Western blot. All experiments were carried out three times. Data are expressed as Mean ± s.e.m