Fig. 1
From: Non-canonical Fzd7 signaling contributes to breast cancer mesenchymal-like stemness involving Col6a1
Fzd7 is associated with mesenchymal phenotype. a and b Heat maps generated from CCLE database (a) and GSE12777 database (b) demonstrated the correlation of FZD7 with mesenchymal-related genes and epithelial-related genes in human BC cell lines. c Expression of Fzd7 was detected in some representative cell lines by Western blot. d and e Expression of Fzd7, Vimentin, E-cadherin, Slug and Zeb1 was detected in MDA-MB-231 cells (d) and Hs578T cells (e) transfected with shCtrl or shFZD7 by Western blot. f Expression of Vimentin was detected in MDA-MB-231 cells transfected with shCtrl or shFZD7 by Immunofluorescence staining. g Morphology of MDA-MB-231 cells transfected with shCtrl or shFZD7 was shown. h Morphology of MCF7 cells transfected with control vector or FZD7 overexpression vector was shown. i Migration of Hs578T cells transfected with shCtrl or shFZD7 was analyzed by Wound healing. j Invasion of MCF7 cells transfected with control vector or FZD7 overexpression vector was analyzed by Transwell. All experiments were carried out three times