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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Discernment between candidate mechanisms for KRAS G13D colorectal cancer sensitivity to EGFR inhibitors

Fig. 3

Competition studies show KRAS G13D is impaired at binding to NF1. a The mean BRET ratio for KRAS-GFP interactions with NF1-NanoLuc for increasing quantities of transfected KRAS constructs. KRAS-flag constructs were also included as a negative control. n = 3. b Assay using Flag-tagged G12V, G12D, or G13D KRAS to evaluate whether each mutant can compete with KRAS G12V-GFP to interact with NF1-Nano-Luc. n = 3. c Assay using Flag-tagged G12V, G12D, or G13D KRAS to evaluate whether each mutant can compete with KRAS G13D-GFP to interact with NF1-NanoLuc. n = 3

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Cell Communication and Signaling

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