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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Rabies virus phosphoprotein P5 binding to BECN1 regulates self-replication by BECN1-mediated autophagy signaling pathway

Fig. 1

Truncated P proteins induce autophagy. a Schematic diagram of the full-length and truncated P proteins. b HEK293T Cells were transfected with the plasmids containing the truncated P genes for 48 h, harvested, and analyzed using western blotting using mouse anti-ATG7, anti-Flag mAb, rabbit anti-LC3A/B, anti-ATG5, anti-BECN1, anti-P62, anti-ULK1, and anti-GAPDH antibodies. c HEK293T cells were cotransfected with GFP-LC3B and the plasmids containing the truncated P genes for 24 h, fixed, and immunostained with mouse anti-Flag antibodies (red), and then visualized using confocal microscopy. DAPI (blue) was used to stain nuclear DNA. Scale bar: 10 μm. d The graph shows the quantification of autophagosomes by taking the average number of dots in 50 cells. Means and SD (error bars) of three independent experiments are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X