Fig. 3

HFD abolishes the increase in hepatic nuclear levels of HIF-1α and LIPIN1 levels observed in Sirt3-deficient mice. a, hepatic nuclear levels of HIF-1α and LIPIN1 from WT and Sirt3^−/− mice fed either a standard chow or a HFD. Data are presented as the mean ± S.D. (n = 6 per group). a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3^−/− mice fed a standard chow. b, nuclear protein levels of HIF-1α and LIPIN1 in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) (Pal) for 24 h. c, hepatic CPT-1α mRNA levels. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, succinate levels in the liver of WT and Sirt3^−/− mice fed either a standard chow or a HFD. a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3^−/− mice fed a standard chow. e, triglyceride levels in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) (Pal) for 24 h in the presence or absence of DMF and/or PX478. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. SIRT3 siRNA cells incubated with palmitate. e, p < 0.05 vs. SIRT3 siRNA cells incubated with palmitate and DMF