Fig. 7

Effect of LOXL1 overexpression on CRC tumorigenesis in vivo. a Left: images of the xenograft tumours of HCT8-N and HCT8-LOXL1 in 4-week-old male nude BALB/c mice, right: corresponding images of haematoxylin-eosin (H-E) staining at magnifications of 25× and 100×. Scale bar: 100 μm. b Growth curve analyses of tumour volumes in HCT8-N/HCT8-LOXL1 cells measured from day 7 to day 19. Tumour width and length were measured with callipers, and growth curves were plotted based on the mean tumour volume at the indicated time points. Data are represented as the mean ± SD; *P < 0.05, ***P < 0.001, n = 3. c Protein extraction from HCT8-N/HCT8-LOXL1 xenograft tumours was immunoblotted with p-YAP (S127), YAP, Flag tag and GAPDH antibodies. d Left: images of xenograft tumours of SW480-N and SW480-LOXL1 in 4-week-old male nude BALB/c mice, right: corresponding images of H-E staining at magnifications of 25× and 100×. Scale bar: 100 μm. e Growth curve analyses of tumour volumes in SW480-N/SW480-LOXL1 cells measured from day 7 to day 19. Tumour width and length were measured with callipers, and growth curves were plotted based on mean tumour volume at the indicated time points. Data are represented as the mean ± SD; *P < 0.05, n = 3. f Protein extracts from SW480-N/SW480-LOXL1 xenograft tumours were immunoblotted with the indicated antibodies. g Left: corresponding images of the lungs of nude BALB/c mice after injection of HCT8-N and HCT8-LOXL1 cells into their tail veins for 8 weeks. (n = 3 mice/group); right: representative H-E images of metastases observed in the lungs of mice. Scale bar: 100 μm. h Left: SW480-N and SW480-LOXL1 cells were injected into the tail vein of nude BALB/c mice and relevant images of metastatic tumours in their livers (n = 3 mice/group); right: representative H-E image of metastases observed in the livers of mice. Scale bar: 100 μm. i-j left: HCT8-N/HCT8-LOXL1 and SW480-N/SW480-LOXL1 cells were injected into the spleens, and metastatic tumours in the livers were assessed 20 days after injection. The relevant images of mouse livers were captured (n = 3 mice/group); right: representative H-E image of metastases observed in the livers of mice. Scale bar: 100 μm