Fig. 6

Effect of LOXL1 △SP expression on the migration and tumorigenesis of HCT8 and SW480 cells in vitro. a Wound healing analysis carried out in HCT8 cells transfected with the control vector, LOXL1 and LOXL1 △SP expression vectors at 0 h, 24 h, and 48 h (upper panel), and the calculation of their wound healing percentages (lower panel). Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. *P < 0.05, **P < 0.01. b Transwell migration and Matrigel invasion assays conducted using the overexpressed cell lines HCT8-N, HCT8-LOXL1 and HCT8-LOXL1 △SP (upper panel) and calculation of the rate of migration/invasion in relevant stable HCT8 cell lines (lower panel). Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. *P < 0.05, **P < 0.01. c A colony formation assay was performed using HCT8 cells after transfection with LOXL1 or LOXL1 △SP. An empty vector was used as the negative control. Upper panel: representative images, lower panel: quantification analysis. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. *P < 0.05, **P < 0.01. d Wound healing analysis carried out on SW480 cells transfected with the LOXL1 expression vector, control vector, and LOXL1 △SP vector at 0 h, 24 h, and 48 h (upper panel), and calculation of their wound healing percentages (lower panel). Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. ***P < 0.001. e Transwell migration and Matrigel invasion assays using the overexpression cell lines SW480-N, SW480-LOXL1, SW480-LOXL1 △SP (upper panel) and calculation of the rate of migration/invasion for relevant SW480 cell lines (lower panel). Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by unpaired t-test. *P < 0.05, ***P < 0.01. f A colony formation assay was carried out using SW480 cells after transfection with LOXL1 or LOXL1 △SP. An empty vector was used as the negative control. Upper panel: representative images, lower panel: quantification analysis. Data from independent experiments are presented as the mean ± SD; statistical significance was assessed by unpaired t-test. **P < 0.01