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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: LOXL1 modulates the malignant progression of colorectal cancer by inhibiting the transcriptional activity of YAP

Fig. 5

LOXL1 activates the Hippo pathway through interaction with MST1/2. a LOXL1 and empty vector were expressed in HEK293T cells for 24 h. Cell lysates were analysed by immunoblotting with the indicated antibodies. b LOXL1, LOXL1 △SP and YAP alone or combined as indicated were transfected into HEK293T cells. The cellular extracts were immunoblotted with the indicated antibodies. c LOXL1 and LOXL1 △SP were transfected into HCT8/SW480 cells for 24 h. Cell lysates were analysed by immunoblotting with the indicated antibodies. d LOXL1 and LOXL1 △SP were transfected into HEK293T cells for 24 h. The cell lysates were analysed using the indicated antibodies. e EGFP or LOXL1-EGFP was cotransfected with MST1-Flag or MST2-Flag in HEK293T cells and subjected to immunoprecipitation with anti-Flag M2 magnetic beads. The immunoprecipitated complexes (upper panels) and whole cell lysates (lower panels) were immunoblotted with the indicated antibodies

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Cell Communication and Signaling

ISSN: 1478-811X