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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Differential regulation of rho GTPases during lung adenocarcinoma migration and invasion reveals a novel role of the tumor suppressor StarD13 in invadopodia regulation

Fig. 2

StarD13 is required for cell motility in lung adenocarcinoma. a A549 cells were transfected with luciferase control siRNA or with StarD13 siRNA (2 oligos) for 72 h. Cells were grown in a monolayer then wounded and left to recover the wound then imaged at the same frame after 48 h (lower micrographs). The graph is a quantitation where wound widths were measured at 11 different points for each wound, and the average rate of wound closure for the luciferase and the StarD13siRNA-transfected cells was calculated in μm/hr. Data are the mean −/+ SEM from 3 independent experiments and *p < 0.05. Scale bar is 100 μm. b The net paths of projected 120 frames from 2 h long time lapse movies of cells (SF268) transfected with luciferase control siRNA or with StarD13 siRNA undergoing random motility in serum (each trace is a cell). The right hand panels for each condition are a close-up trace of a cell area and a net path projected over 2 h. Graph is a quantitation of the cell speed for the time lapse movies in μm/min. Data are the mean −/+ SEM from 15 cells (from 3 independent experiments/10 movies/condition/experiment) and *p < 0.05. Scale bar is 20 μm

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