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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Reverse transcriptase inhibition potentiates target therapy in BRAF-mutant melanomas: effects on cell proliferation, apoptosis, DNA-damage, ROS induction and mitochondrial membrane depolarization

Fig. 2

SPV122 + MAPKi induce DNA damage, mitochondrial membrane depolarization and ROS production in M14 cells. a Immunofluorescence analyses have been performed to quantify nuclear p-H2A.X as a marker of DSBs upon exposure to the aforementioned combinatorial regimens for 24 h. Scale bars: 50 μm; 40x magnification. Heat map has been plotted to quantify the number of foci per cell. b Mitochondrial membrane depolarization has been assessed by FACS analyses after 48 h of exposure of M14 cells to encorafenib, MEK162 and/or SPV122 (left panels). Data have been plotted to assess the % of depolarized cells (right part). c M14 cells treated as described above have been tested for ROS production by FACS analyses

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Cell Communication and Signaling

ISSN: 1478-811X