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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Inhibition of αvβ3 integrin impairs adhesion and uptake of tumor-derived small extracellular vesicles

Fig. 2

SEVs adhere to ECM components and support MDA-MB-231 cell adhesion. a Representative epifluorescence images of CFSE-labeled-SEV adhesion to fibronectin and collagen coating. Treated (DisBa-01500 nM) and untreated (CTRL) groups are compared. Scale bar: 100 μm (whole images); 10 μm (zoomed images). b Quantitation of SEV adhesion to fibronectin and collagen. **** p < 0.0001; * p < 0.05. c Western blot analysis of integrin subunits, α5, β1, α2, β3, and αv and ECM proteins, FN and COL on isolated SEVs and LEVs. d Schematic cartoon of cell adhesion assay to EV coating. e Representative images of Calcein AM-labeled MDA cell adhesion to well bottom, SEVs, LEV and FN coating comparing cell adhesion in control (CTRL) and DisBa-01 (100 nM) treated conditions. f Calcein AM fluorescence intensity of adherent cells. Comparison of adhesion in different coating and effect of DisBa-01 at 100 nM. * p < 0.05; *** p < 0.001; **** p < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X