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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Inhibition of αvβ3 integrin impairs adhesion and uptake of tumor-derived small extracellular vesicles

Fig. 1

Validation of cancer cell-derived EV isolation and characterization. a Representative trace and video acquisition snapshot from nanoparticle tracking analysis of small and large EVs. Both traces show vesicles within a typical size profile. b Transmission electron microscopy of density gradient-purified SEVs and LEVs. Yellow arrows point to representative EVs. Scale bar: 500 nm (large image) and 100 nm (zoomed images). c Western blotting for the EV markers CD63, Flotillin and Alix across 12 iodixanol density gradient fractions, large vesicles (LEV) and whole cell lysate (WCL) of the EV donor cells. Calnexin (CANX) antibody was used as a negative control

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