Fig. 5
From: Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells
PAR-3-induced Tiam1 activation after Thy-1/CD90 treatment requires Syndecan-4. Syndecan-4silenced DI TNC1 astrocytes (siSynd4) or control transfected cells (siCTRL) were stimulated with Thy-1-Fc-Protein A (Thy-1) or treated with TRAIL-R2-Fc-Protein A (TRAIL) for 60 min. Cells were fixed, and stained for GST to detect GST-Rac1G15A (green) and for Tiam1 (red). The white rectangle indicates the zoomed area. The heat color map indicates levels of total Tiam1 co-localization with nucleotidefree mutant of Rac1 (GST-Rac1G15A) at the membrane, as indicative of Tiam1 activation. Scale bar = 10 μm