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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: The critical role of dysregulated Hh-FOXM1-TPX2 signaling in human hepatocellular carcinoma cell proliferation

Fig. 6

FOXM1 promotes HCC cell proliferation through TPX2. a. WB analysis of Huh7 cells infected with FOXM1 overexpression and TPX2 knockdown lentiviral vectors. b. Comparison of the proliferative ability of Lv-control + sh-Con, Lv-control + sh-TPX2, Lv-FOXM1 + sh-Con, and Lv-FOXM1 + sh-TPX2 in Huh7 cells using the EdU assay. Scale bar, 100 μm. c. The ratio of EdU-positive cells was quantified using the ImageJ software (n = 3). d. Growth curves of Lv-control + sh-Con, Lv-control + sh-TPX2, Lv-FOXM1 + sh-Con, and Lv-FOXM1 + sh-TPX2 of Huh7 cells. e-h. Comparison of the proliferative ability (f) and plate colony formation assay (h) of Lv-control + sh-Con, Lv-control + sh-TPX2, Lv-FOXM1 + sh-Con, and Lv-FOXM1 + sh-TPX2 in Huh7 cells. Scale bar, 100 μm. Soft agar formation (g) and plate colony formation (I) were quantified using the ImageJ software. i. Huh7 cells stably expressing FOXM1 + shTPX2 were subjected to cell cycle analysis (left panel) and fraction of cells in each phase quantified (right panel). j. Quantification (%) of multinucleated Huh7 cells stably expressing FOXM1 + shTPX2. Data was represented as mean ± SD of three independent experiments. *, p < 0.05; **, p < 0.01

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