Fig. 4

FOXM1 directly activates TPX2 transcription. a. Consensus sequence of FOXM1-binding site, sourced from MotifMap [33]. b. Predicted FOXM1-binding sites within the genomic sequence adjacent to the transcription start site of TPX2. The underlined sequences with red lines are core sequence of FOXM1-binding sites. c. Schematic diagram showing candidate FOXM1-binding site within the TPX2 promoter, the full-length luciferase reporter construct, and its deletion variants with different FOXM1-binding sites. d. Dual luciferase assay of TPX2 promoter-luciferase reporters in FOXM1-expressing or control HepG2 cells. e-g. Dual luciferase assay of sets of TPX2 promoter truncation-luciferase reporters in FOXM1-expressing or control HepG2 cells. h. ChIP assay in Huh7 cells to detect FOXM1 binding to the TPX2 promoter. Data was represented as mean ± SD of three independent experiments. **, p < 0.01