Fig. 1

TPX2 expression is regulated by the Hh signaling pathway. a. Venn diagrams of differentially expressed genes (DEGs) in Huh7 and HepG2 cells after treating with GANT61 versus genes enriched in “Cell Cycle” gene set. b. Representative candidate genes derived from Venn diagrams in Fig. 1a were represented in a heat map. Red signal denotes higher expression and blue signal denotes lower expression. Gene names marked in red are previously reported genes regulated by FOXM1. c. Hep3B cells were treated with GANT61 (10 ~ 20 μM) for 48 h and harvested for real-time PCR analysis with the indicated primers. d. Hep3B cells were treated with GANT61 (left panel) or cyclopamine (right panel) (10 ~ 20 μM) for 48 h and harvested for WB analysis with the indicated antibodies. e. Hep3B cells were treated with cyclopamine (10 ~ 20 μM) for 48 h and harvested for real-time PCR analysis with the indicated primers. f-g. Hep3B cells transfected with shRNA-control or shRNA-GLI2 was harvested for real-time PCR analysis with the indicated primers (f) and for WB analysis with the indicated antibodies (g). h-i. Huh7cells transfected with Lv-control or Lv-GLI2A were subjected to real-time PCR analysis with the indicated primers (h) as well as WB analysis with the indicated antibodies (i). j. Huh7 cells incubated with or without N-Shh were subjected to real-time PCR analysis with the indicated primers. k. Huh7 cells incubated with N-Shh for 0, 12, and 24 h were harvested for WB analysis with the indicated antibodies. Data was shown as mean ± SD (n = 3). *, p < 0.05; **, p < 0.01