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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Molecular basis of carrageenan-induced cytokines production in macrophages

Fig. 7

Cg-induced colitis in mice depends on NLRP3 inflammasome. The naive WT and Nlrp3−/−, Casp1/11−/− and Pycard−/− mice received Cg 5% or saline daily by gavage. a Body weight was measured daily up to 20 days in all groups. After 20 days of treatment, colonoscopy examination was performed. b, c Representative colonoscopy images from the splenic flexure region, descending colon, rectum and Colitis colonoscopy scores were determined comparing the control group, WT, Casp1/11 −/−, Pycard −/− and Nlrp3−/− mice. d Representative H&E and immunohistochemistry stained for MPO+ cells from colon sections 20 days after Cg (5%) treatment. e Histological severity scores were determined comparing the control group, WT, Casp1/11 −/−, Pycard −/− and Nlrp3−/− mice 20 days after Cg (5%) treatment. f Infiltration of neutrophils into the distal colon wall was estimated using myeloperoxidase immunostaining. g MPO activity was determined in the colon tissue from control group, WT, Casp1/11 −/−, Pycard −/− and Nlrp3−/− mice 20 days after Cg (5%) treatment. h The colon samples were also used for the quantification of IL-1β contents by Elisa. Data are represent the mean ± SD. (*p < 0.05; **p < 0.01; and ***p < 0.001)

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